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KMID : 0364820140500030179
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Korean Journal of Microbiology
2014 Volume.50 No. 3 p.179 ~ p.184
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Iron Containing Superoxide Dismutase of Streptomyces subrutilus P5 Increases Bacterial Heavy Metal Resistance by Sequestration
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Kim Jae-Heon
Han Kwang-Yong
Jung Ho-Jin
Lee Jung-Nam
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Abstract
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Mitigation of heavy metal toxicity by iron containing superoxide dismutase (FeSOD) of Streptomyces subrutilus P5 was investigated. For E. coli DH5α, the survival rate in the presence of 0.1 mM lead ions was only 7% after 120 min; however, with the addition of 0.1 μM of purified native FeSOD the survival rate increased to 39%. This detoxification effect was also shown with 0.01 mM copper ions (survival increased from 6% to 50%), and the effect was stronger than with the use of EDTA. E. coli M15[pREP4] producing 6xHis-tagged FeSOD was constructed, and this showed an increase in survival rates throughout the incubation time; in the presence of 0.1 mM lead ions,the final increase at 60 min was from 3% to 19%. The FeSOD absorbed about 123 g-atom lead per subunit; therefore, we suggest that FeSOD could sequestrate toxic heavy metals to enhance bacterial survival against heavy metal contamination.
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KEYWORD
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Escherichia coli, Streptomyces subrutilus, heavy metals, resistance, sequestration, superoxide dismutase
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